Erik Strandberg

Phone: +49-(0)721-608-8700, Fax: +49-(0)721-608-4823
Email: Erik.Strandberg@ioc.uka.de

 

Research project: Solid state NMR studies of membrane active peptides.

Read more: <Solid state NMR>, <Antimicrobial peptides>, <PGLa>.

 

Research interests: Membrane peptides and proteins, solid state NMR spectroscopy, lipid-peptide interactions, peptide-peptide interactions, structure calculations.

Positions

 

2003-

Institut für Biologische Grenzflechen (IBG), Forschungszentrum Karlsruhe, BioNMR Group, PostDoc

2000-2002

Dept. of Biochemistry of Membranes, Universiteit Utrecht, Netherlands, PostDoc

1994-1999

Dept. of Biophysical Chemistry, Umeċ universitet, Sweden, Ph.D. student.

 

Publications

Accepted articles:

23. Parvesh Wadhwani and Erik Strandberg (2008). “Structure analysis of membrane-active peptides using 19F-labeled amino acids and solid state NMR”. In: Iwao Ojima and Takeo Taguchi (Editors), “Fluorine in Bioorganic and Medicinal Chemistry”. London: Blackwell Publishing. (Review)

 

Published articles:

 

22. Erik Strandberg, Nathalie Kanithasen, Deniz Tiltak, Jochen Bürck, Parvesh Wadhwani, Olaf Zwernemann, and Anne S. Ulrich. Biochemistry 47, 2601-2616. (2008).  ”Solid-state NMR analysis comparing the designer-made antibiotic MSI-103 with its parent peptide PGLa in lipid bilayers”.

 

21. Parvesh Wadhwani, Pierre Tremouilhac, Erik Strandberg, Sergii Afonin, Stephan L. Grage, Marco Ieronimo, Marina Berditsch, and Anne S. Ulrich. (2007). “Using fluorinated amino acids for structure analysis of membrane-active peptides by solid-state 19F-NMR”. In: V. A. Soloshonok, K. Mikami, T. Yamazaki, J. T. Welch and J. F. Honek (Editors). “Current fluoroorganic chemistry: new synthetic directions, technologies, materials, and biological applications”. Washington, DC: American Chemical Society. p 431-446. (Review)

 

20. Erik Strandberg, Deniz Tiltak, Marco Ieronimo, Nathalie Kanithasen, Parvesh Wadhwani, and Anne S. Ulrich. Pure and Applied Chemistry, 79, 717-728.  (2007). “Influence of C-terminal amidation on the antimicrobial and hemolytic activities of cationic a-helical peptides”.

 

19. Pierre Tremouilhac, Erik Strandberg, Parvesh Wadhwani, and Anne S. Ulrich. Journal of Biological Chemistry 281, 32089-32094 (2006). “Synergistic transmembrane alignment of the antimicrobial heterodimer PGLa/magainin”.

 

18. Pierre Tremouilhac, Erik Strandberg, Parvesh Wadhwani, and Anne S. Ulrich. Biochimica et Biophysica Acta 1758, 1330–1342 (2006). “Conditions affecting the re-alignment of the antimicrobial peptide PGLa in membranes as monitored by solid-state 2H-NMR”.

 

17. Erik Strandberg, Parvesh Wadhwani, Pierre Tremouilhac, Ulrich H. N. Dürr, and Anne S. Ulrich. Biophysical Journal 90, 1676-1686 (2006). “Solid-state NMR analysis of the PGLa peptide orientation in DMPC bilayers: structural fidelity of 2H-labels versus high sensitivity of 19F-NMR”.

 

16. Anne S. Ulrich, Parvesh Wadhwani, Ulrich H. N. Dürr, Sergii Afonin, Ralf W. Glaser, Erik Strandberg, Pierre Tremouilhac, Carsten Sachse, Marina Berditchevskaia, and Stephan L. Grage. (2006). “Solid-state 19F-nuclear magnetic resonance analysis to membrane-active peptides”. In: Ayyalusamy Ramamoorthy (Editor). “NMR spectroscopy of biological solids”. Boca Raton: CRC Press. p. 215-236. (Review)

 

15. Ralf W. Glaser, Carsten Sachse, Ulrich H. N. Dürr, Parvesh Wadhwani, Sergii Afonin, Erik Strandberg and Anne S. Ulrich. Biophysical Journal 88, 3392-3397

 (2005). “Concentration-dependent re-alignment of the antimicrobial peptide PGLa in lipid membranes observed by solid state 19F-NMR”.

 

14. Barbara Hoff, Erik Strandberg, Anne S. Ulrich, Peter Tieleman and Clemens Posten. Biophysical Journal, 88, 1818-1827 (2005). “2H NMR study and molecular dynamics simulation of the location, alignment, and mobility of pyrene in POPC bilayers”.

 

13. Erik Strandberg and Anne Ulrich. Concepts in Magnetic Resonance Part A, 23A, 89-120  (2004). “NMR methods to study membrane-active antimicrobial peptides”. (Review)

 

12. Erik Strandberg, Suat Özdirekcan, Dirk T. S. Rijkers, Patrick C. A. van der Wel, Roger E. Koeppe II, Rob M. J. Liskamp and J. Antoinette Killian, Biophysical Journal, 86, 3709-3721 (2004). “Tilt angles of transmembrane model peptides in oriented and non-oriented lipid bilayers as determined by 2H solid state NMR”.

 

11. Erik Strandberg and J. Antoinette Killian. FEBS Letters, 544, 69-73, (2003). “Snorkeling of lysine side chains in transmembrane helices: how easy can it get?”

 

10. Patrick C. van der Wel, Erik Strandberg, J. Antoinette Killian and Roger E. Koeppe, II. Biophysical Journal , 83, 1479-1488 (2002). ”Geometry and intrinsic tilt of a tryptophan-anchored transmembrane alpha-helix determined by 2H NMR”.

 

9. Erik Strandberg, Sven Morein, Dirk T. Rijkers, Rob M. Liskamp, Patrick C. A. van der Wel and J. Antoinette Killian. Biochemistry, 41, 7190-7198 (2002). “Lipid dependence of membrane anchoring properties and snorkeling behavior of aromatic and charged residues in transmembrane peptides.”

 

8. Erik Strandberg, Tobias Sparrman and Göran Lindblom. Advances in Colloid and Interface Science, 89-90, 239-261 (2001). “Phase diagrams of systems with cationic a-helical membrane-spanning model peptides and dioleolyphosphatidylcholine”.

 

7. Tomas Byström, Erik Strandberg, Frank A. Kovacs, Timothy A. Cross and Göran Lindblom. Biochimica et Biophysica Acta, 1509, 335-345 (2000). “Influence of transmembrane peptides on bilayers of phosphatidylcholines with different acyl chain lengths studied by solid-state NMR”.

 

6. Greger Orädd, Ann-Sofie Andersson, Leif Rilfors, Göran Lindblom, Erik Strandberg and Per E. Andrén, Biochimica et Biophysica Acta, 1468, 329-344 (2000). “a-Metylene ordering of acyl chains differs in glucolipids and phosphatidylglycerol from Acholeplasma laidlawii membranes: 2H-NMR quadrupole splittings from individual lipids in mixed bilayers”.

 

5. Erik Strandberg and Per-Olof Westlund. Journal of Magnetic Resonance, 137, 333-344 (1999). "Paramagnetic Proton Nuclear Spin Relaxation Theory of Low-Symmetry Complexes for Electron Spin Quantum Number S=5/2".

 

4. André Pampel, Erik Strandberg, Göran Lindblom and Frank Volke. Chemical Physics Letters, 287, 468-474 (1998). "High resolution NMR on cubic lyotropic liquid crystalline phases".

 

3. Sven Morein, Erik Strandberg, J Antoinette Killian, Stefan Persson, Gösta Arvidsson, Roger E Koeppe II and Göran Lindblom, Biophysical Journal, 73, 3078-3088 (1997). "Influence of Membrane-Spanning a-Helical Peptides on the Phase Behavior of the Dioleoylphosphatidylcholine/Water System".

 

2. Erik Strandberg, Johan Kördel, Ronny Lundin, Thomas Wehler and Göran Widmalm. Journal of Peptide Research, 49, 254-260 (1997). "Nuclear magnetic resonance studies of the C-terminal human growth hormone fragment I179-C182-[SS]-C189-P191 and the related trisulphide peptide I179-C182-[SSS]-C189-P191".

 

1. Erik Strandberg and Per-Olof Westlund. Journal of Magnetic Resonance, Series A, 122, 179-191 (1996). " 1H NMRD Profile and ESR Lineshape Calculation for an Isotropic Electron Spin System with S = 7/2. A Generalized Modified Somolon-Bloembergen-Morgan Theory for Non-Extreme Narrowing Conditions".

 

 

Theses:

Erik Strandberg, Ph. D. thesis, Umeċ University (2000). ”Membrane lipids and peptides studied by nucelar magnetic resonance spectroscopy”.

 

Erik Strandberg, Teknologie licentiat thesis, Umeċ University (1998). ”A generalized modified Solomon-Bloembergen-Morgan theory of paramagnetic spin relaxation”.

 

 

Submitted articles:

 

-

 

 

 

Projects in progress:

 

Erik Strandberg, Parvesh Wadhwani, and Anne S. Ulrich, “NMR studies of membrane-active antimicrobial peptides”. Peptide Science (Invited review)

 

Deniz Tiltak, Erik Strandberg, Parvesh Wadhwani, Pierre Tremouilhac, Nathalie Kanithasen, Olaf Zwernemann, Marco Ieronimo, Marina Berditchevskaia, Anne S. Ulrich. “Lipid effects on the orientation of the antimicrobial peptide MSI-103 in membranes”

 

Pierre Tremouilhac, Erik Strandberg, Parvesh Wadhwani, and Anne S. Ulrich. “ 2H NMR study of magainin 2 in  lipid bilayers”

 

 

 

Talks at scientific conferences

 

Upcoming talk:

-

Previous talk:

 

Erik Strandberg, “Concentration-dependent re-alignment of antimicrobial peptides in lipid bilayers studied by solid state NMR”. Membrane Biophysics of Antimicrobial Peptides, Ann Arbor, Michigan, USA, 24-25 September, 2005.

 

 

Other talks:

 

Sergii Afonin, Pavel K. Mykhailiuk, Stephan L. Grage, Jochen Bürck, Ulrich Sternberg, Parvesh Wadhwani, Igor V. Komarov, Anne S. Ulrich. (Presented by Erik Strandberg.) “Structural investigations of the cell-penetrating peptide SAP in lipid membranes”, 52nd Annual Meeting of the Biophysical Society & 16th International Biophysics Congress, Long Beach, California, USA, 2-6 February, 2008.

 

Erik Strandberg, “Solid state NMR methods to investigate peptide-membrane interactions”, Workshop Forschungszentrum Karlsruhe and Forschungszentrum Jülich, Wiesbaden, 19-20 October 2007.

 

Erik Strandberg, Anne S. Ulrich, “Re-alignment and self-assembly of antimicrobial peptides in membranes studied by solid state NMR”, Schwerpunkt Meeting Biomimetic antimicrobial macromolecules, Hamburg, 22 November, 2006.

 

Erik Strandberg, “Orientation of antimicrobial peptides in lipid bilayers studied by solid state NMR”, invited lecture, Department of Biophysical Chemistry, Umeċ University, Umeċ, Sweden, 31 October 2006.

 

 

Poster presentations at scientific conferences

 

Upcoming presentations:

 

Erik Strandberg, Santi Esteban-Martin, Jesús Salgado, Anne S. Ulrich. ”Orientation and dynamics of membrane bound peptides studied with solid state NMR”, Bijvoet 20th Anniversary Symposium, Utrecht, The Netherlands, 7-8 April, 2008.

 

Erik Strandberg, Parvesh Wadhwani, Pierre Tremouilhac, Santi Esteban-Martin, Jesús Salgado, Anne S. Ulrich. ”Orientation and dynamics of membrane-bound peptides studied with solid state NMR”, SBNet Swedish Strucutral Biology Conference, Tällberg, Sweden,13-16 June, 2008.

 

 

 

 

Previous presentations:

 

Erik Strandberg, Santi Esteban-Martin, Jesús Salgado, Anne S. Ulrich. ”Orientation of membrane bound peptides studied with solid state NMR”, 52nd Annual Meeting of the Biophysical Society & 16th International Biophysics Congress, Long Beach, California, USA, 2-6 February, 2008.

 

Erik Strandberg, Pierre Tremouilhac, Deniz Tiltak, Parvesh Wadhwani, Sergii Afonin, Stephan Grage, Marco Ieronimo, Anne S. Ulrich. “Orientation of antimicrobial peptides in lipid membranes stidued by solid-state 2H- and 19F-NMR”, EUROMAR Magnetic Resonance Conference, Tarragona, Spain, 1-6 July, 2007.

 

Erik Strandberg, Pierre Tremouilhac, Parvesh Wadhwani, Anne S. Ulrich. “Toroidal wormhole in membranes formed by antimicrobial peptides observed by solid state NMR”, 51st Annual Meeting of the Biophysical Society, Baltimore, Maryland, USA, 3-7 March, 2007.

 

Erik Strandberg, Pierre Tremouilhac, Parvesh Wadhwani, Anne S. Ulrich. “Membrane pores formed by antimicrobial peptides observed by solid state NMR”, 6th Annual Surface and Colloid Symposium on Lipid-Peptide Interactions and Biological Function, Lund, Sweden, 15-17 November, 2006.

 

Erik Strandberg, Pierre Tremouilhac, Parvesh Wadhwani, Anne S. Ulrich. “Alignment of membrane-bound peptides studied by solid state 2H- and 19F-NMR”, 22nd International Conference on Magnetic Resonance in Biological Systems, Göttingen, Germany, 20-25 August, 2006.

 

Erik Strandberg, Parvesh Wadhwani, Pierre Tremouilhac, Anne S. Ulrich. “Re-alignment of membrane-bound antimicrobial peptides studied by solid state 2H- and 19F-NMR”, NMR Symposium at the 48th Rocky Mountain Conference on Analytical Chemistry, Breckenridge, Colorado, USA, 24-27 July, 2006.

 

Erik Strandberg, Parvesh Wadhwani, Pierre Tremouilhac, Nathalie Kanithasen, Jakob Schaefer, Anne S. Ulrich. “Dimerization of magainin family antimicrobial peptides in lipid bilayers studied by 2H, 19F, and 15N solid-state NMR“, 18th European Experimental Nuclear Magnetic Resonance Conference/EUROMAR, Veldhoven, The Netherlands, 3-8 July, 2005.

 

Erik Strandberg, Barbara Hoff, D. Peter Tieleman, Clemens Posten, Anne S. Ulrich. ”2H-NMR study and molecular dynamics simulation of the location, alignment and mobility of pyrene in POPC bilayers”, 49th Annual Meeting of the Biophysical Society, Long Beach, California, USA, 12-16 February, 2005.

 

Erik Strandberg, Parvesh Wadhwani, Pierre Tremouilhac, Carsten Sachse, Ralf Glaser, Anne Ulrich. “19F, 2H, and 15N SSNMR studies of the antimicrobial peptide PGLa in lipid bilayers”, The 45th Experimental Nuclear Magnetic Resonance Conference (ENC), Asilomar Conference Center, Pacific Grove, California, USA, 18-23 April, 2004.

 

Erik Strandberg, Parvesh Wadhwani, Pierre Tremouilhac, Carsten Sachse, Ralf Glaser, Anne Ulrich. “Alignment of the antimicrobial peptide PLGa in membranes“, 3rd Alpine Conference on Solid-State NMR, Chamonix, France, 14-18 September, 2003.

 

Erik Strandberg, Suat Özdirekcan, Dirk T. S. Rijkers, J. Antoinette Killian, “Structure and dynamics of a-helical membrane-spanning model peptides”, 16th European Experimental Nuclear Magnetic Resonance Conference, Prague, Czech Republic, 9-14 June, 2002.

 

Erik Strandberg, Dirk T. S. Rijkers, and J. Antoinette Killian , “Structure and dynamics of a-helical membrane-spanning model peptides determined in unoriented phospholipid systems using solid state NMR”, 46th Annual Meeting of the Biophysical Society, San Francisco, California, USA, 23-27 February, 2002.

 

Erik Strandberg, Sven Morein, Patrick C. A. van der Wel, Roger E. Koeppe II, J. Antoinette Killian, “The snorkeling behaviour of side chains in model transmembrane peptides is dependent on the lipid headgroup composition”, 45th Annual Meeting of the Biophysical Society, Boston, Massachussetts, USA, 17-21 February 2001.

 

Erik Strandberg, Patrick C. A. van der Wel, J. Antoinette Killian, “Interactions of transmembrane peptides and lipids studied with solid state NMR”, Anno 2000: A lipid milestone, Utrecht, The Netherlands, 26-28 October, 2000.

 

Erik Strandberg, Patrick C. A. van der Wel, J. Antoinette Killian, “Interactions of transmembrane peptides and lipids studied with solid state NMR”, International workshop “The future of solid state NMR in biology”, Leiden, The Netherlands, 4-8 October 2000.

 

Erik Strandberg, Sven Morein, Göran Lindblom, “NMR studies of a membrane-spanning a-helical peptide”, 29th Ampere-13th ISMAR Conference, Berlin, Germany, 2-7 August, 1998.

 

Erik Strandberg, Göran Lindblom, “Influence of a membrane-spanning a-helical peptide on the phase behaviour of the DOPC/water system”, 42nd Annual Meeting of the Biophysical Society. Kansas City, Missouri, USA, 22-26 February, 1998.

 

Erik Strandberg, Sven Morein, Tomas Byström, Göran Lindblom, J. Antoinette Killian, Gösta Arvidson, Roger E. Koeppe II, “Influence of putative membrane-spanning a-helical peptides on the phase behavior of phospholipid/water systems”; Erik Strandberg, Per-Olof Westlund, “Theoretical calculations of NMRD profiles and ESR lineshapes for micro-heterogenous systems with spin quantum number S=5/2”, 13th Scandinavian Symposium on Surface Chemistry, Umeċ, Sweden, 24-26 August, 1997.

 

Erik Strandberg, Per-Olof Westlund, “Theoretical calculations of NMRD profiles and ESR lineshapes for micro-heterogenous systems with spin quantum number S=5/2”, 7th Chianti Workshop on Magnetic Resonance, Nuclear and Electronic Relaxation, San Miniato (Pisa), Italy, 25-31 May, 1997.

 

Erik Strandberg, Sven Morein, Göran Lindblom, J. Antoinette Killian, Roger E. Koeppe II, “Influence of putative membrane-spanning a-helical peptides on the phase behavior of dioleoylphosphatidylcholine/water systems”, Joint British/Australian Biophysical Societies' Conference. Satellite Meeting to the IOPAB Congress in Amsterdam, Oxford, Great Britain, 18-20 August, 1996.

 

Erik Strandberg, Thomas Wehler, Johan Kördel, Ronny Lundin, Göran Widmalm, “A conformational study of two cyclic peptides by NMR spectroscopy, distance geometry and restrained molecular dynamics”, Nordic NMR Symposium, Stockholm, Sweden, 16-19 May, 1995.

 

Last updated 3 April 2008.